Regulatory

Part:BBa_I712004:Experience

Designed by: Andrej Ondracka   Group: iGEM07_Ljubljana   (2007-10-19)


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Applications of BBa_I712004

Usage and Biology

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edit by sysu-medicine

We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.


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Fig.1 The plasmid we used for transfection.


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Fig.2 Transfection cells observed under a fluorescence microscope.


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Fig.3 Fluorescent quantitation by Image J(P<0.001).

UNIQb71a458ea77ddcb3-partinfo-00000000-QINU UNIQb71a458ea77ddcb3-partinfo-00000001-QINU